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Antimicrobial Effect of Chitosan Nanoparticles and Allium Species on Mycobacterium tuberculosis and Several Other Microorganisms.

Authors :
Olivas-Flores, Jocelyn
Chávez-Méndez, José Román
Castillo-Martínez, Nydia Alejandra
Sánchez-Pérez, Héctor Javier
Serrano-Medina, Aracely
Cornejo-Bravo, José Manuel
Source :
Microorganisms; Aug2024, Vol. 12 Issue 8, p1605, 14p
Publication Year :
2024

Abstract

This study evaluates the antimicrobial efficacy of chitosan nanoparticles (CNPs), varying in size, against clinical isolates of Mycobacterium tuberculosis (MTB), E. coli, S. aureus, E. faecalis, and C. albicans, as well as the antimicrobial effects of aqueous extracts and lyophilized powders of Allium (garlic) species. CNPs were synthesized through ionotropic gelation and characterized by Z potential, hydrodynamic diameter (dynamic light scattering, DLS), and SEM. Aqueous garlic extracts were prepared via decoction. We assessed antimicrobial activity using disk diffusion and broth microdilution methods; in addition, a modified agar proportion method in blood agar was used for antimicrobial activity against MTB. CNPs inhibited MTB growth at 300 μg for 116.6 nm particles and 400 μg for 364.4 nm particles. The highest antimicrobial activity was observed against E. faecalis with nanoparticles between 200 and 280 nm. Allium sativum extract produced inhibition for C. albicans at 100 μg. The results indicate that CNPs possess significant antimicrobial properties against a range of pathogens, including MTB, at high concentrations. On the other hand, aqueous Allium sativum extracts exhibited antimicrobial activity. Nonetheless, due to their instability in solution, the use of lyophilized Allium sativum powder is preferable. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20762607
Volume :
12
Issue :
8
Database :
Complementary Index
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
179377960
Full Text :
https://doi.org/10.3390/microorganisms12081605