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GhWRKY40 Interacts with an Asparaginase GhAP D6 Involved in Fiber Development in Upland Cotton (Gossypium hirsutum L.).
- Source :
- Genes; Aug2024, Vol. 15 Issue 8, p979, 14p
- Publication Year :
- 2024
-
Abstract
- Fiber quality improvement is a primary goal in cotton breeding. Identification of fiber quality-related genes and understanding the underlying molecular mechanisms are essential prerequisites. Previously, studies determined that silencing the gene GhWRKY40 resulted in longer cotton fibers; however, both the underlying mechanisms and whether this transcription factor is additionally involved in the regulation of cotton fiber strength/fineness are unknown. In the current study, we verified that GhWRKY40 influences the fiber strength, fiber fineness, and fiber surface structure by using virus-induced gene silencing (VIGS). Potential proteins that may interact with the nucleus-localized GhWRKY40 were screened in a yeast two-hybrid (Y2H) nuclear-system cDNA library constructed from fibers at 0, 10, and 25 days post-anthesis (DPA) in two near-isogenic lines differing in fiber length and strength. An aspartyl protease/asparaginase-related protein, GhAP<subscript>D6</subscript>, was identified and confirmed by Y2H and split-luciferase complementation assays. The expression of GhAP<subscript>D6</subscript> was approximately 30-fold higher in the GhWRKY40-VIGS lines at 10 DPA and aspartyl protease activity was significantly upregulated in the GhWRKY40-VIGS lines at 10–20 DPA. This study suggested that GhWRKY40 may interact with GhAP<subscript>D6</subscript> to regulate fiber development in cotton. The results provide a theoretical reference for the selection and breeding of high-quality cotton fibers assisted by molecular technology. [ABSTRACT FROM AUTHOR]
- Subjects :
- TRANSCRIPTION factors
COTTON fibers
GENE silencing
NANOTECHNOLOGY
SURFACE structure
Subjects
Details
- Language :
- English
- ISSN :
- 20734425
- Volume :
- 15
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 179353992
- Full Text :
- https://doi.org/10.3390/genes15080979