Researchers' Work from Nahda University Focuses on Antibiotics (Various Approaches for Appraisal of the Greenness Profile and Whiteness Assessment of Chromatographic Methods for Determination of Spiramycin, Metronidazole, and Toxic Impurity:...).

Researchers from Nahda University in Bani Suwayf, Egypt have developed two eco-friendly chromatographic methods for determining the concentrations of Spiramycin (SPR) and Metronidazole (MTR) in pharmaceutical preparations. The first method involves TLC-densitometry using silica gel plates and a mixture of methanol, ethyl acetate, and ammonium hydroxide solution as the eluent. The second method uses RP-HPLC with a Bridge C 18 column and a mobile phase of methanol and acidic water. These methods were validated and found to be accurate and efficient for routine quality control assessments of the drugs. The researchers also conducted computational analysis to predict the toxicity profile and pharmacokinetic properties of a potentially harmful impurity, 4-nitroimidazole (IMP). The environmental impact of the methods was assessed using various metrics, and the quality of the methods was evaluated in terms of whiteness. The research concludes that these established approaches are effective for evaluating the efficacy of pharmaceutical preparations. [Extracted from the article]