14-3-3 promotes sarcolemmal expression of cardiac CaV1.2 and nucleates isoproterenol-triggered channel super-clustering.

A preprint abstract from biorxiv.org discusses the role of a protein called 14-3-3 in regulating the expression and activity of the L-type Ca2+ channel (CaV1.2) in cardiomyocytes. The study found that 14-3-3 interacts with CaV1.2 in a phosphorylation-dependent manner and promotes its trafficking, clustering, and activity during beta-adrenergic stimulation. The researchers also observed that 14-3-3 overexpression increased basal CaV1.2 cluster size and Ca2+ currents in ventricular myocytes, while inhibition of 14-3-3 abrogated the isoproterenol-stimulated augmentation of sarcolemmal CaV1.2 expression and currents. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]