Back to Search
Start Over
Dry blood spots as a sampling strategy to identify insulin resistance markers during a dietary challenge.
- Source :
- Genes & Nutrition; 8/29/2024, Vol. 19 Issue 1, p1-9, 9p
- Publication Year :
- 2024
-
Abstract
- This study aimed to identify markers of postprandial dysglycemia in the blood of self-described healthy individuals using dry blood spots (DBS) as a sampling strategy. A total of 54 volunteers, including 31 women, participated in a dietary challenge. They consumed a high-fat, high-sugar mixed meal and underwent multiple blood sampling over the course of 150 min to track their postprandial responses. Blood glucose levels were monitored with a portable glucometer and individuals were classified into two groups based on the glucose area under the curve (AUC): High-AUC (H-AUC) and Low-AUC (L-AUC). DBS sampling was performed at the same time points as the assessment of glycemia using Whatman 903 Protein Saver filter paper. A gas chromatography-mass spectrometry-based metabolite profiling was conducted in the DBS samples to assess postprandial changes in blood metabolome. Higher concentrations of metabolites associated with insulin resistance were observed in individuals from the H-AUC group, including sugars and sugar-derived products such as fructose and threonic acid, as well as organic acids and fatty acids such as succinate and stearic acid. Several metabolites detected in the GC-MS analysis remained unidentified, indicating that other markers of hyperglycemia remain to be discovered in DBS. Based on these observations, we demonstrated that the use of DBS as a non-invasive and inexpensive sampling tool allows the identification of metabolites markers of dysglycemia in the postprandial period. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15558932
- Volume :
- 19
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Genes & Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 179326453
- Full Text :
- https://doi.org/10.1186/s12263-024-00752-7