Adsorption/desorption behavior and purification process optimization of theaflavins on macroporous resin.

Theaflavins, as products of tea fermentation, exhibit significant applications in the fields of functional food, medicine, and others. This study aimed to screen ideal resin, further investigate its adsorption mechanisms, and optimize the dynamic purification process of ideal resin. Through static adsorption/desorption studies, LX-20B resin with great adsorption and desorption ability was screened from six macroporous resins. The adsorption mechanism of LX-20B resin was studied through static adsorption kinetics and thermodynamic experiments. The results showed that the adsorption of TFs on LX-20B resin corresponded to the pseudo-second-order kinetic model and Langmuir kinetic model, indicating that the adsorption is a high-temperature spontaneous, and monolayer adsorption process. After studying the adsorption mechanism of LX-20B resin, the process parameters for purifying TFs using LX-20B resin were further optimized. At a sample concentration of 150 mg/mL and a sample loading flow rate of 1.0 BV/h, sequential elution with 3.0 BV water, 4.0 BV of 30% (v/v) ethanol, and 2.0 BV of 60% (v/v) ethanol at a flow rate of 1.0 BV/h led to a significant increase in TFs purity from 14.28 ± 0.05% to 62.48 ± 0.37%, with a recovery rate of 52.98 ± 2.01%. The purity was 4.4 times greater than that of the crude material. [ABSTRACT FROM AUTHOR]