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OsALKBH9‐mediated m6A demethylation regulates tapetal PCD and pollen exine accumulation in rice.

Authors :
Tang, Jun
Lei, Dekun
Yang, Junbo
Chen, Shuyan
Wang, Xueping
Huang, Xiaoxin
Zhang, Shasha
Cai, Zhihe
Zhu, Shanshan
Wan, Jianmin
Jia, Guifang
Source :
Plant Biotechnology Journal; Sep2024, Vol. 22 Issue 9, p2410-2423, 14p
Publication Year :
2024

Abstract

Summary: The N6‐methyladenosine (m6A) mRNA modification is crucial for plant development and stress responses. In rice, the male sterility resulting from the deficiency of OsFIP37, a core component of m6A methyltransferase complex, emphasizes the significant role of m6A in male fertility. m6A is reversible and can be removed by m6A demethylases. However, whether mRNA m6A demethylase regulates male fertility in rice has remained unknown. Here, we identify the mRNA m6A demethylase OsALKBH9 and demonstrate its involvement in male fertility regulation. Knockout of OsALKBH9 causes male sterility, dependent on its m6A demethylation activity. Cytological analysis reveals defective tapetal programmed cell death (PCD) and excessive accumulation of microspores exine in Osalkbh9‐1. Transcriptome analysis of anthers shows up‐regulation of genes involved in tapetum development, sporopollenin synthesis, and transport pathways in Osalkbh9‐1. Additionally, we demonstrate that OsALKBH9 demethylates the m6A modification in TDR and GAMYB transcripts, which affects the stability of these mRNAs and ultimately leads to excessive accumulation of pollen exine. Our findings highlight the precise control of mRNA m6A modification and reveal the pivotal roles played by OsALKBH9‐mediated m6A demethylation in tapetal PCD and pollen exine accumulation in rice. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14677644
Volume :
22
Issue :
9
Database :
Complementary Index
Journal :
Plant Biotechnology Journal
Publication Type :
Academic Journal
Accession number :
179090758
Full Text :
https://doi.org/10.1111/pbi.14354