Upscaling the production of sterile male mosquitoes with an automated pupa sex sorter.

Effective mosquito population suppression has been repeatedly demonstrated in field trials through the release of male mosquitoes to induce sterile mating with wild females using the incompatible insect technique (IIT), the sterile insect technique (SIT), or their combination. However, upscaling these techniques requires a highly efficient and scalable approach for the sex separation of mass-reared mosquitoes to minimize the unintentional release of females, which can lead to either population replacement or biting nuisance, a major bottleneck up to now. Here, we report the successful development of an automated mosquito pupa sex sorter that can effectively separate large numbers of males from females for population suppression of Aedes aegypti, A. albopictus, and Culex quinquefasciatus. The male production capacity of the automated sex sorter was increased by ~17-fold compared with manual sex separation with the Fay-Morlan sorter and enabled one person to separate 16 million males per week. With ~0.5% female contamination, the produced males exhibited high flight ability and mating performance. The field trial demonstrates that the quality of A. albopictus males produced using the automated sex sorter is suitable for inducing population suppression. These results indicate that the automated sex sorter offers the potential to upscale IIT and SIT against mosquito vectors for disease control. Editor's summary: Several techniques have been developed to fight mosquito-borne diseases, including the release of sterile or incompatible male mosquitoes into the wild to reduce population growth. However, sorting mosquitoes by sex is a laborious process. Gong et al. have now developed an automated mosquito pupa sex sorter, which was tested on three mosquito species and shown to increase the production of males 17-fold when compared with a manual separation process. A field trial in Guangzhou, China showed that the process was capable of producing sufficient incompatible males without reducing their quality, which led to the suppression of wild populations of mosquitoes. —Amos Matsiko [ABSTRACT FROM AUTHOR]