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Coexistence of blaIMP−4 and blaSFO−1 in an IncHI5B plasmid harbored by tigecycline-nonsusceptible Klebsiella variicola strain.
- Source :
- Annals of Clinical Microbiology & Antimicrobials; 3/6/2024, Vol. 23 Issue 1, p1-8, 8p, 1 Diagram, 2 Charts, 1 Graph
- Publication Year :
- 2024
-
Abstract
- Background Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the bla<subscript>IMP−4</subscript> and bla<subscript>SFO−1</subscript> genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics. Methods Species identification was conducted using MALDI-TOF/MS. PCR identification confirmed the presence of the blaIMP−4 and bla<subscript>SFO−1</subscript> genes. Antibiotic resistance phenotype and genomic characteristics were detected by antimicrobial susceptibility testing and whole-genome sequencing. Plasmid characterization was carried out through S1-PFGE, conjugation experiments, Southern blot, and comparative genomic analysis. Results K. variicola 4253 belonged to ST347, and demonstrated resistance to broad-spectrum β-lactamase drugs and tigecycline while being insensitive to imipenem and meropenem. The bla<subscript>IMP−4</subscript> and bla<subscript>SFO−1</subscript> genes harbored on the plasmid p4253-imp. The replicon type of p4253-imp was identified as IncHI5B, representing a multidrug-resistant plasmid capable of horizontal transfer and mediating the dissemination of drug resistance. The bla<subscript>IMP−4</subscript> gene was located on the In809-like integrative element (Intl1-bla<subscript>IMP−4</subscript>-aacA4-catB3), which circulates in Acinetobacter and Enterobacteriaceae. Conclusions This study reports the presence of a strain of K. variicola, which is insensitive to tigecycline, carrying a plasmid harboring bla<subscript>IMP−4</subscript> and bla<subscript>SFO−1</subscript>. It is highly likely that the strain acquired this plasmid through horizontal transfer. The bla<subscript>IMP−4</subscript> array (Intl1-bla<subscript>IMP−4</subscript>-aacA4-catB3) is also mobile in Acinetobacter and Enterobacteriaceae. So it is essential to enhance clinical awareness and conduct epidemiological surveillance on multidrug-resistant K. variicola, conjugative plasmids carrying bla<subscript>IMP−4</subscript>, and the In809 integrative element. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14760711
- Volume :
- 23
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Annals of Clinical Microbiology & Antimicrobials
- Publication Type :
- Academic Journal
- Accession number :
- 179002427
- Full Text :
- https://doi.org/10.1186/s12941-024-00680-9