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Antibody gene features associated with binding and functional activity in malaria vaccine-derived human mAbs.
- Source :
- NPJ Vaccines; 8/10/2024, Vol. 9 Issue 1, p1-7, 7p
- Publication Year :
- 2024
-
Abstract
- The impact of adjuvants on malaria vaccine-induced antibody repertoire is poorly understood. Here, we characterize the impact of two adjuvants, Alhydrogel® and AS01, on antibody clonotype diversity, binding and function, post malaria vaccination. We expressed 132 recombinant anti-Pfs230D1 human monoclonal antibodies (mAbs) from participants immunized with malaria transmission-blocking vaccine Pfs230D1, formulated with either Alhydrogel® or AS01. Anti-Pfs230D1 mAbs generated by Alhydrogel® formulation showed higher binding frequency to Pfs230D1 compared to AS01 formulation, although the frequency of functional mAbs was similar between adjuvant groups. Overall, the AS01 formulation induced anti-Pfs230D1 functional antibodies from a broader array of germline sequences versus the Alhydrogel® formulation. All mAbs using IGHV1-69 gene from the Alhydrogel® cohort bound to recombinant Pfs230D1, but did not block parasite transmission to mosquitoes, similar to the IGHV1-69 mAbs isolated from the AS01 cohort. These findings may help inform vaccine design and adjuvant selection for immunization with Plasmodium antigens. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANTIBODY diversity
MALARIA vaccines
MONOCLONAL antibodies
MALARIA
GERM cells
Subjects
Details
- Language :
- English
- ISSN :
- 20590105
- Volume :
- 9
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- NPJ Vaccines
- Publication Type :
- Academic Journal
- Accession number :
- 178970509
- Full Text :
- https://doi.org/10.1038/s41541-024-00929-6