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Antibody gene features associated with binding and functional activity in malaria vaccine-derived human mAbs.

Authors :
Coelho, Camila H.
Marquez, Susanna
Nguemwo Tentokam, Bergeline C.
Berhe, Anne D.
Miura, Kazutoyo
Rao, Vishal N.
Long, Carole A.
Doumbo, Ogobara K.
Sagara, Issaka
Healy, Sara
Kleinstein, Steven H.
Duffy, Patrick E.
Source :
NPJ Vaccines; 8/10/2024, Vol. 9 Issue 1, p1-7, 7p
Publication Year :
2024

Abstract

The impact of adjuvants on malaria vaccine-induced antibody repertoire is poorly understood. Here, we characterize the impact of two adjuvants, Alhydrogel® and AS01, on antibody clonotype diversity, binding and function, post malaria vaccination. We expressed 132 recombinant anti-Pfs230D1 human monoclonal antibodies (mAbs) from participants immunized with malaria transmission-blocking vaccine Pfs230D1, formulated with either Alhydrogel® or AS01. Anti-Pfs230D1 mAbs generated by Alhydrogel® formulation showed higher binding frequency to Pfs230D1 compared to AS01 formulation, although the frequency of functional mAbs was similar between adjuvant groups. Overall, the AS01 formulation induced anti-Pfs230D1 functional antibodies from a broader array of germline sequences versus the Alhydrogel® formulation. All mAbs using IGHV1-69 gene from the Alhydrogel® cohort bound to recombinant Pfs230D1, but did not block parasite transmission to mosquitoes, similar to the IGHV1-69 mAbs isolated from the AS01 cohort. These findings may help inform vaccine design and adjuvant selection for immunization with Plasmodium antigens. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20590105
Volume :
9
Issue :
1
Database :
Complementary Index
Journal :
NPJ Vaccines
Publication Type :
Academic Journal
Accession number :
178970509
Full Text :
https://doi.org/10.1038/s41541-024-00929-6