Virtual Screening of a-glucosidase and a-amylase Inhibitors from Soybean Peptides and Its Activity Validation in Vitro.

The objective: To delay the digestion of carbohydrates by inhibiting the activity of carbohydrate digestive enzymes, thereby preventing and controlling the effects of diabetes has become a research hotspot across multiple fields. j8 Conglycinin is the most abundant functional protein in soy protein, but it is unknown whether the peptides produced after gastrointestinal digestion possess the activity to inhibit carbohydrate digestive enzymes. Method: Conglycinin was subjected to simulated gastrointestinal digestion, and glucosidase inhibitory peptides and amylase inhibitory peptides were selected through virtual screening and ADMET prediction, and the inhibitory effects of the peptides on " --glucosi-dase and " --amylase were tested. Results: ! --Conglycinin was virtually digested into 95 small molecular peptides by pepsin, trypsin, and chymotrypsin; eight "--glucosidase inhibitory peptides and "--amylase inhibitory peptides were selected; it was found that hydrogen bonds and electrostatic interactions play an important role in the binding of peptides with glucosidase and amylase; in vitro verification found that the tetrapeptide EASY has a strong inhibitory effect on glucosidase, with an IC0 value of 208.6 g/mL, and no obvious inhibitory effect on amylase was observed. Conclusion: The glucosidase inhibitory peptide EASY possesses the activity to inhibit carbohydrate digestive enzymes and may become a potential inhibitor for controlling diabetes. [ABSTRACT FROM AUTHOR]