Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison.

Introduction: Centella is an important genus in the Apiaceae family. It includes Centella asiatica, which has significant edible and medicinal values. However, this species is easily confused due to its similar morphological traits to Hydrocotyle umbellata, hindering its utilization in the consumer and pharmacological industries. Objective: The study aims to differentiate these two closely related plant species using reliable methods of confirming the authenticity of natural herbal medicines. Methods: Our work mainly focuses on the basic morphological characteristics, chemical markers, genetic fingerprints, and their biological responses. Results: The plants can be clearly differentiated using their leaf shapes, stipules, petioles, inflorescences, and fruit structures. Although the phytochemical compositions of the C. asiatica extract were similar to that of H. umbellata which included flavonoids, tannins, and saponins important to the plant's ability to reduce inflammation and promote healing of wounds, the H. umbellata extract showed significantly higher toxicity than that of C. asiatica. High‐performance liquid chromatography analysis was used to identify chemical fingerprints. The result revealed that C. asiatica had major triterpene glycoside constituents including asiaticoside, asiatic acid, madecassoside, and madecassic acid, which have a wide range of medicinal values. In contrast, triterpenoid saponins were not identified in H. umbellata. Furthermore, using SCoT1–6 primers was possible to effectively and sufficiently created a dendrogram which successfully identified the closeness of the plants and confirmed the differences between the two plant species. Conclusion: Therefore, differentiation can be achieved through the combination of morphometrics, molecular bioactivity, and chemical analysis. Centella asiatica (L.) Urban and Hydrocotyle umbellata Linn are easily confused due to their similar morphological characteristics and habitats. Leaf shape, flower, and fruit characteristics are morphological markers to differentiate them. HPLC and SCoT markers are powerful tools to differentiate closely related plant species. C. asiatica had major triterpene glycoside constituents. They exhibit promising properties for anti‐inflammation and wound healing. The high toxicity of H. umbellata extract, however, raises serious concerns about its use in food, medicine, and cosmeceutical. [ABSTRACT FROM AUTHOR]