Effective strategies for creating self-compatible B. rapa by introgression of mutated SRK and SCR/SP11 genes from B. napus.

This article discusses effective strategies for creating self-compatible Brassica rapa (Chinese cabbage) plants by introducing mutated SRK and SCR/SP11 genes from Brassica napus (rapeseed). Self-incompatibility (SI) is a common trait in Brassica vegetable crops, but it hinders the development and propagation of inbred lines. The manipulation of SI genes, such as SRK and SCR/SP11, can lead to the creation of self-compatible materials. The researchers used CRISPR/Cas9 technology to mutate the BnSRK-1 gene in B. napus and then employed distant hybridization to introduce the mutated gene into B. rapa, resulting in the generation of self-compatible materials. This research has important implications for improving the breeding process of Brassica vegetable crops. [Extracted from the article]