Elite Genotype Characterization and Genetic Structure Analysis of the Medicinal Tree Archidendron clypearia (Jack) I. C. Nielsen Using Microsatellite Markers.

Archidendron clypearia (Jack) I. C. Nielsen is an important tree species for traditional medicine purposes such as anti-inflammatory, detoxification, uric acid control and neuro-protection. Here, a set of 15 microsatellite markers were used to fingerprint 248 elite genotypes from six origins of A. clypearia and investigate the genetic structure of these genotypes. A total of 170 alleles were amplified. The elite genotypes showed moderate genetic variability, with mean observed heterozygosity (H_o) and expected heterozygosity (H_e) being 0.651 and 0.699, respectively. Each of the elite genotypes could be fingerprinted uniquely. The cumulative probability of identity (PI) and the cumulative probability of paternity exclusion (PE) were 2.25 × 10⁻¹² and 0.999992, respectively, demonstrating the extremely high power of the markers for genotype identification. In STRUCTURE and clustering analyses, the same origin of elite genotypes tended to be grouped in a sub-population and clustered closely, but some genotypes from different geographic origins were mixed, suggesting a low level of genetic differentiation between the origins. These results can contribute to the protection and management of the elite genotype resources of A. clypearia and the comprehensive understanding of its genetic structure. [ABSTRACT FROM AUTHOR]