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A Type of Ferrocene-Based Derivative FE-1 COF Material for Glycopeptide and Phosphopeptide Selective Enrichment.
- Source :
- Journal of Functional Biomaterials; Jul2024, Vol. 15 Issue 7, p185, 17p
- Publication Year :
- 2024
-
Abstract
- In this work, a new type of FE-1 COF material is prepared by a reversible imine condensation reaction with diaminoferrocene and diaminodiformaldehyde as materials. The material is connected by imine bonds to form a COF skeleton, and the presence of plenty of nitrogen-containing groups gives the material good hydrophilicity; the presence of metal Fe ions provides the material application potential in the enrichment of phosphopeptides. According to the different binding abilities of N-glycopeptide and phosphopeptide on FE-1 COF, it can simultaneously enrich N-glycopeptide and phosphopeptide through different elution conditions to realize its controllable and selective enrichment. Using the above characteristics, 18 phosphopeptides were detected from α-casein hydrolysate, 8 phosphopeptides were detected from β-casein hydrolysate and 21 glycopeptides were detected from IgG hydrolysate. Finally, the gradual elution strategy was used; 16 phosphopeptides and 19 glycopeptides were detected from the α-casein hydrolysate and IgG hydrolysate. The corresponding glycopeptides and phosphopeptides were identified from the human serum. It proves that the FE-1 COF material has a good enrichment effect on phosphopeptides and glycopeptides. [ABSTRACT FROM AUTHOR]
- Subjects :
- PHOSPHOPEPTIDES
GLYCOPEPTIDES
CONDENSATION reactions
METAL ions
SKELETON
Subjects
Details
- Language :
- English
- ISSN :
- 20794983
- Volume :
- 15
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Journal of Functional Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 178694433
- Full Text :
- https://doi.org/10.3390/jfb15070185