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Genetic Variants of the Receptor Activator Nuclear of κB Ligand Gene Increase the Risk of Rheumatoid Arthritis in a Mexican Mestizo Population: A Case–Control Study.

Authors :
Arturo, Nava-Valdivia Cesar
Ivan, Gamez-Nava Jorge
Betsabe, Contreras-Haro
Emilio, Perez-Guerrero Edsaul
Yussef, Esparza-Guerrero
Alejandra, Rodriguez-Jimenez Norma
Tonatiuh, Gonzalez-Heredia
Alejandra, Villagomez-Vega
Ismael, Nuño-Arana
Elena, Totsuka-Sutto Sylvia
Manuel, Ponce-Guarneros Juan
Heriberto, Jacobo-Cuevas
Gerardo, Alvarez-Ayala Efren
Laura, Gonzalez-Lopez
Miriam, Saldaña-Cruz Ana
Source :
Genes; Jul2024, Vol. 15 Issue 7, p907, 10p
Publication Year :
2024

Abstract

The Receptor Activator Nuclear of κB Ligand (RANKL) plays an important function in immune responses, activating osteoclast cells and unchanged bone resorption, which in turn leads to bone erosion and inflammation. Genetic variants in the promoter region of the RANKL gene could lead to a higher risk of rheumatoid arthritis (RA). Objective: To assess the association of rs9533155 (-693C>G) and rs9533156 (-643T>C) genetic variants with RA risk. Methods: A case–control study was carried out. A total of 94 patients with RA (RA group) and 134 subjects without any rheumatologic disease (control group) were included. Genetic DNA was extracted from peripheral white blood cells (leukocytes). Genetic variant rs9533155 (-693C>G) was screened by an approach based on Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP), while rs9533156 (-643T>C) was screened using quantitative polymerase chain reaction (qPCR) with TaqMan probes. RANKL serum levels were measured by ELISA. Results: For rs9533155 (-693C>G), the polymorphic homozygous genotype frequencies (CC) were higher in the RA group (p = 0.006). Individuals carrying the risk genotype presented higher levels of serum RANKL. Carriers of the polymorphic homozygous genotype in the dominant model (CC vs. CG + GG) had an increased risk of developing RA (OR: 1.8, 95% CI 1.04 to 3.1). No association between rs9533156 (-643T>C) and the haplotypes with RA risk was observed. Conclusion: The rs9533155 (-693C>G) genetic variant exhibits a potential role in RA risk. The studied population had no association with the rs9533156 (-643T>C) genetic variant. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20734425
Volume :
15
Issue :
7
Database :
Complementary Index
Journal :
Genes
Publication Type :
Academic Journal
Accession number :
178689385
Full Text :
https://doi.org/10.3390/genes15070907