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Single-cell analysis of human PBMCs in healthy and type 2 diabetes populations: dysregulated immune networks in type 2 diabetes unveiled through single-cell profiling.

Authors :
Doeon Gu
Jinyeong Lim
Kyung Yeon Han
In-Ho Seo
Jae Hwan Jee
Soo Jin Cho
Yoon Ho Choi
Sung Chul Choi
Jang Hyun Koh
Jin-Young Lee
Mira Kang
Dong-Hyuk Jung
Woong-Yang Park
Source :
Frontiers in Endocrinology; 2024, p1-10, 10p
Publication Year :
2024

Abstract

Abnormalities in glucose metabolism that precede the onset of type 2 diabetes (T2D) activate immune cells, leading to elevated inflammatory factors and chronic inflammation. However, no single-cell RNA sequencing (scRNA-seq) studies have characterized the properties and networks of individual immune cells in T2D. Here, we analyzed peripheral blood mononuclear cells (PBMCs) from non-diabetes and T2D patients by scRNA-seq. We found that CD14 monocytes in T2D patients were in a pro-inflammatory state and intermediate monocytes expressed more MHC class II genes. In T2D patients, cytotoxic CD4 T cells, effector memory CD8 T cells, and gd T cells have increased cytotoxicity and clonal expansion. B cells were characterized by increased differentiation into intermediate B cells, plasma cells, and isotype class switching with increased expression of soluble antibody genes. These results suggest that monocytes, T cells, and B cells could interact to induce chronic inflammation in T2D patients with pro-inflammatory characteristics. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16642392
Database :
Complementary Index
Journal :
Frontiers in Endocrinology
Publication Type :
Academic Journal
Accession number :
178674981
Full Text :
https://doi.org/10.3389/fendo.2024.1397661