Molecular Epidemiology of Human Parainfluenza Virus Type 3 in Children With Acute Respiratory Tract Infection in Hangzhou.

Background: Since the outbreak of COVID‐19, China has undertaken a variety of preventative and control measures, effectively reducing the incidence of numerous infectious diseases among the pediatric population in Hangzhou. We aim to investigate the genetic and epidemiological characteristics of Human parainfluenza virus‐3 (HPIV‐3) in pediatric patients during this period. Methods: A total of 1442 pharyngeal swab samples were collected from outpatients and inpatients with a diagnosis of acute respiratory tract infections (ARTIs) from November 2020 to March 2021. HPIV‐3 was detected by quantitative real time polymerase chain reaction (qRT‐PCR). The L gene of HPIV‐3 positive samples was amplified and sequenced. Results: Among 1442 children with ARTI, the positive rate of HPIV‐3 was 7.07% (102/1442). The positive detection rate was the highest in the 6‐month to 1‐year age group. Coinfection was observed in 36 HPIV‐3‐positive samples (35.29%, 36/102), and adenovirus (ADV) was the most common coinfecting virus (63.89%, 23/36). The L gene of 48 HPIV‐3 positive samples was sequenced. The nucleotide sequence analysis showed high consistency (92.10%–99.40%), and all strains belonged to C3a. Conclusions: During study periods, the positive detection rate of HPIV‐3 among children is high, and the highest proportion of coinfection was observed in HPIV‐3 mixed ADV infection. Phylogenetic analysis revealed that the nucleotide sequence of the L gene of HPIV‐3 was highly consistent, and the main epidemic strain in this area was the C3a subtype. [ABSTRACT FROM AUTHOR]