Biosensors in environmental analysis of microplastics and heavy metal compounds – A review on current status and challenges.

Biosensors (biological sensors) are a promising alternative to standard analytical techniques for testing and monitoring in the environment, among others, heavy metals. Biological materials that detect heavy metals include enzymes, bacteria, fungi, and plant and animal cells. The paper aimed to evaluate the possibilities of biosensor usage in environmental analysis, considering an easy and environmentally friendly analysis of individual compounds (e.g., heavy metals) or their mixtures in various samples directly in the environment, as well as continuous monitoring of these compounds. The work considers biosensors' characteristics, classification, and operation principles. Attention is paid to the possibility of using bioluminescent bacteria as biological material in a biosensor. Examples of the study of heavy metals using various biological sensors were given. It also indicates the possibility of using biosensors to determine microplastics by applying specific hormones (estrogens) receptor use and peptides as a solution or incorporating them into living microbial cells. Additionally, support was given to the use of biosensors to monitor plastics' degradation in the water environment using recombinant whole-cell microbial biosensors as detectors for plastic monomers such as acrylic acid monomers. The application of the biosensors highlighted in the paper is to shorten the time of toxicity studies by using new indicators or acceptors. However, several challenges must be overcome, such as cell viability and activity. A continuous water-monitoring system that can handle a severely toxic sample has yet to be developed in toxicity monitoring [ABSTRACT FROM AUTHOR]