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Integrated network pharmacology and bioinformatics to identify therapeutic targets and molecular mechanisms of Huangkui Lianchang Decoction for ulcerative colitis treatment.
- Source :
- BMC Complementary Medicine & Therapies; 7/23/2024, Vol. 24 Issue 1, p1-17, 17p
- Publication Year :
- 2024
-
Abstract
- Background: Huangkui Lianchang Decoction (HLD) is a traditional Chinese herbal formula for treating ulcerative colitis (UC). However, its mechanism of action remains poorly understood. The Study aims to validate the therapeutic effect of HLD on UC and its mechanism by integrating network pharmacology, bioinformatics, and experimental validation. Methods: UC targets were collected by databases and GSE19101. The active ingredients in HLD were detected by ultra-performance liquid chromatography-tandem mass spectrometry. PubChem collected targets of active ingredients. Protein–protein interaction (PPI) networks were established with UC-related targets. Gene Ontology and Kyoto Encyclopedia (KEGG) of Genes and Genomes enrichment were analyzed for the mechanism of HLD treatment of UC and validated by the signaling pathways of HLD. Effects of HLD on UC were verified using dextran sulfate sodium (DDS)-induced UC mice experiments. Results: A total of 1883 UC-related targets were obtained from the GSE10191 dataset, 1589 from the database, and 1313 matching HLD-related targets, for a total of 94 key targets. Combined with PPI, GO, and KEGG network analyses, the signaling pathways were enriched to obtain IL-17, Toll-like receptor, NF-κB, and tumor necrosis factor signaling pathways. In animal experiments, HLD improved the inflammatory response of UC and reduced UC-induced pro-inflammatory factors such as Tumor Necrosis Factor Alpha (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6). HLD suppressed proteins TLR4, MyD88, and NF-κB expression. Conclusions: This study systematically dissected the molecular mechanism of HLD for the treatment of UC using a network pharmacology approach. Further animal verification experiments revealed that HLD inhibited inflammatory responses and improved intestinal barrier function through the TLR4/MyD88/NF-κB pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- INFLAMMATION prevention
CHINESE medicine
NF-kappa B
COMPUTER-assisted molecular modeling
BIOLOGICAL models
WEIGHT loss
LIQUID chromatography-mass spectrometry
GENOMICS
CARRIER proteins
RESEARCH funding
FECES
GASTROINTESTINAL hemorrhage
TISSUES
T-test (Statistics)
PHARMACEUTICAL chemistry
HERBAL medicine
STATISTICAL sampling
ENZYME-linked immunosorbent assay
ULCERATIVE colitis
CELLULAR signal transduction
TOLL-like receptors
DESCRIPTIVE statistics
PHYTOCHEMICALS
BIOINFORMATICS
MICE
GENE expression
IMMUNOHISTOCHEMISTRY
COLON (Anatomy)
ANIMAL experimentation
HISTOLOGICAL techniques
WESTERN immunoblotting
ONE-way analysis of variance
MOLECULAR structure
GASTROINTESTINAL agents
MOLECULAR biology
BIOAVAILABILITY
DATA analysis software
COMPARATIVE studies
INTERLEUKINS
TUMOR necrosis factors
DRUG dosage
PHARMACODYNAMICS
DRUG administration
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 178589586
- Full Text :
- https://doi.org/10.1186/s12906-024-04590-3