Back to Search
Start Over
Polyoxometalate functionalized magnetic metal–organic framework with multi-affinity sites for efficient enrichment of phosphopeptides.
- Source :
- Analytical & Bioanalytical Chemistry; Aug2024, Vol. 416 Issue 19, p4289-4299, 11p
- Publication Year :
- 2024
-
Abstract
- The reasonable design of metal–organic framework (MOF)–derived nanomaterial has important meaning in increasing the enrichment efficiency in the study of protein phosphorylation. In this work, a polyoxometalate (POM) functionalized magnetic MOF nanomaterial (Fe<subscript>3</subscript>O<subscript>4</subscript>@MIL-125-POM) was designed and fabricated. The nanomaterial with multi-affinity sites (unsaturated metal sites and metal oxide clusters) was used for the enrichment of phosphopeptides. Fe<subscript>3</subscript>O<subscript>4</subscript>@MIL-125-POM had high-efficient enrichment performance towards phosphopeptides (selectivity, a mass ratio of bovine serum albumin/α-casein/β-casein at 5000:1:1; sensitivity, 0.1 fmol; satisfactory repeatability, ten times). Furthermore, Fe<subscript>3</subscript>O<subscript>4</subscript>@MIL-125-POM was employed to enrich phosphopeptides from non-fat milk digests, saliva, serum, and A549 cell lysate. The enrichment results illustrated the great potential of Fe<subscript>3</subscript>O<subscript>4</subscript>@MIL-125-POM for efficient identification of low-abundance phosphopeptides. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 416
- Issue :
- 19
- Database :
- Complementary Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 178530083
- Full Text :
- https://doi.org/10.1007/s00216-024-05365-y