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Evaluation of coagulation and platelet activation state and function in heartworm-infected dogs.

Authors :
Fraser, Carisa
Wallace, Mandy L.
Moorhead, Andrew
Tarigo, Jaime
Brainard, Benjamin M.
Source :
Veterinary Clinical Pathology; Jun2024, Vol. 53 Issue 2, p186-195, 10p
Publication Year :
2024

Abstract

Background: Enhanced platelet responses have been demonstrated in heartworm-infected (HWI) dogs; however, the cause and clinical implications of altered platelet function have not been fully elucidated. Objective: This study evaluated platelet function in HWI dogs. Methods: Anticoagulated whole blood collected from eight HWI and eight uninfected dogs was evaluated using turbidometric platelet aggregometry, a platelet function analyzer (PFA-100), a total thrombus analysis system (T-TAS), tissue factor-activated and tissue plasminogen activator modified thromboelastography (TF- and tPA-TEG), CBC, von Willebrand Factor activity, and fibrinogen concentrations. Platelet activation state and the presence of reticulated platelets were assessed via flow cytometric expression of P-selection (CD-62P) and thiazole orange staining. Results: Platelet aggregation responses to adenosine diphosphate (ADP, 10 μM) or collagen (20 μg/mL), PFA-100 closure times, and T-TAS occlusion times did not differ between groups. TEG values TF-R, tPA-R, TF-K, and TF-LY60 were decreased (P= .025, P= .047, P = .038, P = .025) and TF-MA, tPA-MA, TF-G, tPA-G and TF-alpha angle were increased (P < .04) in HWI dogs. HWI dogs had higher fibrinogen concentrations (465.6 ± 161 mg/dL vs 284.5 ± 38 mg/dL, P = .008) and eosinophil counts (0.686 ± 0.27 × 10³/μL vs 0.267 ± 0.20 × 10³/μL, P = .003). There was no difference in hematocrit, activation state, or percent of reticulated platelets. Non-activated reticulated platelets exhibited higher CD62P expression compared with mature platelets. Conclusions: Chronic canine heartworm disease was accompanied by hypercoagulability, hyperfibrinogenemia, and decreased fibrinolysis. Enhanced platelet activation was not identified in this group of HWI dogs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
02756382
Volume :
53
Issue :
2
Database :
Complementary Index
Journal :
Veterinary Clinical Pathology
Publication Type :
Academic Journal
Accession number :
178521585
Full Text :
https://doi.org/10.1111/vcp.13358