Spatial top-down proteomics for the functional characterization of human kidney (Updated July 5, 2024).

This article discusses a new method for characterizing the proteome, or the complete set of proteins, in human kidney tissue. The researchers used laser capture microdissection, nanoliter-scale sample preparation, and mass spectrometry imaging to identify and analyze different proteoforms, which are variations of proteins resulting from genetic differences and modifications. They focused their analysis on specific functional units of the kidney and found differential abundance of certain proteoforms between these units. This technology has the potential to provide insights into the causes and mechanisms of diseases by studying the proteomic landscape. However, it is important to note that this research has not yet undergone peer review. [Extracted from the article]