Back to Search Start Over

Production of a new chitinase from Nocardiopsis halophila TN-X8 utilizing bio-waste from the blue swimming crab: enzyme characterization and immobilization.

Authors :
Mechri, Sondes
Jabeur, Fadoua
Bessadok, Boutheina
Moumnassi, Sara
Idrissi Yahyaoui, Meryem
Mannani, Nysrine
Asehraou, Abdeslam
Mensi, Fethi
Vita, Stefano
D'Amore, Paolo
Di Bella, Calogero
Lo Monaco, Daniela
Abousalham, Abdelkarim
Sadok, Saloua
Le Roes-Hill, Marilize
Jaouadi, Bassem
Source :
Environmental Science & Pollution Research; Jul2024, Vol. 31 Issue 32, p45217-45233, 17p
Publication Year :
2024

Abstract

In accordance with the framework of the Circular Blue Bioeconomy in the Mediterranean region, the objective of this study was to evaluate the biotransformation of blue swimming crab (Portunus segnis) residues obtained from the port of Sfax by an extracellular chitinase produced by Nocardiopsis halophila strain TN-X8 isolated from Chott El Jerid (Tozeur, Tunisia). From the analysis of multiple extremophilic Actinomycetota, it was determined that strain TN-X8 exclusively utilized 60 g/L of raw blue swimming crab as its carbon and energy source, achieving a chitinase activity of approximately 950 U/mL following a 6-day incubation period at 40 °C. Pure chitinase, designated as ChiA-Nh30, was obtained after heat treatment, followed by ammonium sulfate fractionation and Sephacryl® S-200 column chromatography. The maximum ChiA-Nh30 activity was observed at pH 3 and 75 °C. Interestingly, compared with cyclohexamidine, ChiA-Nh30 showed a good antifungal effect against four pathogenic fungi. Furthermore, when using colloidal chitin as substrate, ChiA-Nh30 demonstrated a higher degree of catalytic efficiency than the commercially available Chitodextrinase®. In addition, ChiA-Nh30 could be immobilized by applying encapsulation and encapsulation-adsorption techniques. The kaolin and charcoal used acted as excellent binders, resulting in improved ChiA-Nh30 stability. For the immobilized ChiA-Nh30, the yield of N-acetyl-d-glucosamine monomers released from 20% (w/v) blue swimming crab residues increased by 3.1 (kaolin) and 2.65 (charcoal) times, respectively. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09441344
Volume :
31
Issue :
32
Database :
Complementary Index
Journal :
Environmental Science & Pollution Research
Publication Type :
Academic Journal
Accession number :
178484192
Full Text :
https://doi.org/10.1007/s11356-024-34088-x