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A Dicyanoisoflurone-based Near-infrared Fluorescence Probe for Highly Sensitive Detection of Hg2+.
- Source :
- Journal of Fluorescence; Jul2024, Vol. 34 Issue 4, p1821-1828, 8p
- Publication Year :
- 2024
-
Abstract
- Due to its high toxicity, long durability, easy absorption by aquatic organisms, and significant bioaccumulation, Hg<superscript>2+</superscript> has caused substantial environmental damage and posed serious threats to human health. Therefore, effective detection of Hg<superscript>2+</superscript> is of utmost importance. In this study, a turn-on fluorescent probe based on dicyanoisoflurone was developed for the detection of Hg<superscript>2+</superscript>. The probe exhibited near-infrared fluorescence signal at 660 nm upon excitation by 440 nm UV light in a mixture of CH<subscript>3</subscript>CN and HEPES buffer (4:1, v/v, 10 mM, pH = 7.5), with selective binding to Hg<superscript>2+</superscript> in a molar ratio of 1:1. This binding event was accompanied by a visible color change from light yellow to orange. By utilizing the enhanced fluorescence signal change, this probe enables highly sensitive analysis and detection of Hg<superscript>2+</superscript> with excellent selectivity (association constant = 1.63 × 10<superscript>4</superscript> M<superscript>− 1</superscript>), large Stokes shift (220 nm), high sensitivity (detection limit as low as 5.6 nM), short reaction time (30 s), and a physiological pH range of 7.5–9.5. The probe was successfully employed for detecting of Hg<superscript>2+</superscript> in real water and living cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10530509
- Volume :
- 34
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Journal of Fluorescence
- Publication Type :
- Academic Journal
- Accession number :
- 178444337
- Full Text :
- https://doi.org/10.1007/s10895-023-03386-x