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The m6A reader SlYTH2 negatively regulates tomato fruit aroma by impeding the translation process.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 7/9/2024, Vol. 121 Issue 28, p1-9, 50p
- Publication Year :
- 2024
-
Abstract
- N<superscript>6</superscript>-methyladenosine (m<superscript>6</superscript>A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m<superscript>6</superscript>A readers. However, whether and how m6A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m<superscript>6</superscript>A reader protein and profiled the binding sites of SlYTH2 at the transcriptome-wide level. SlYTH2 undergoes liquid-liquid phase separation and promotes RNA-protein condensate formation. The target mRNAs of SlYTH2, namely m<superscript>6</superscript>A-modified SlHPL and SlCCD1B associated with volatile synthesis, are enriched in SlYTH2-induced condensates. Through polysome profiling assays and proteomic analysis, we demonstrate that knockout of SlYTH2 expedites the translation process of SlHPL and SlCCD1B, resulting in augmented production of aroma-associated volatiles. This aroma enrichment significantly increased consumer preferences for CRISPR-edited fruit over wild type. These findings shed light on the underlying mechanisms of m<superscript>6</superscript>A in plant RNA metabolism and provided a promising strategy to generate fruits that are more attractive to consumers. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 121
- Issue :
- 28
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 178410834
- Full Text :
- https://doi.org/10.1073/pnas.2405100121