Nanoparticles co-delivering siRNA and mRNA for simultaneous restoration and silencing of gene/protein expression in vitro and invivo.

A preprint abstract from biorxiv.org discusses the potential of RNA-based agents, such as siRNA, miRNA, and mRNA, to manipulate gene expression and proteins for therapeutic purposes, particularly in cancer treatment. To overcome the limitations of RNA agents, researchers have developed nanoparticles (NPs) to deliver functional mRNA or siRNA into cells. This proof-of-concept study focuses on co-delivery NPs containing Luc-mRNA and siRNA-GFP, which were found to effectively impact the expression of two genes/proteins in vitro and in a mouse model of triple-negative breast cancer. The study suggests that these NPs have the potential to be used in RNA-based anticancer therapeutics and pave the way for multitargeted approaches in complex diseases like cancer. [Extracted from the article]