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New synthesis of oligosaccharides modelling the M epitope of the Brucella O-polysaccharide.

Authors :
Tsvetkov, Yury E.
Volkov, Timur M.
Eremin, Sergei A.
Sklyarov, Oleg D.
Kulakov, Yuri K.
Krylov, Vadim B.
Nifantiev, Nikolay E.
Romano, Maria Rosaria
Source :
Frontiers in Chemistry; 2024, p1-14, 14p
Publication Year :
2024

Abstract

Brucellosis is a dangerous zoonotic disease caused by bacteria of the genus Brucella. Diagnosis of brucellosis is based on the detection in animal and human sera of antibodies to the O-polysaccharide of Brucella lipopolysaccharide. The currently employed serodiagnosis of brucellosis relies on the use of the Brucella O-polysaccharide as a diagnostic antigen. However, the existence of bacterial species, which also express O-polysaccharides structurally similar to that of Brucella, may decrease the specificity of the brucellosis detection due to false-positive test results. It has been shown that the efficiency of the test can be significantly improved by using synthetic oligosaccharides that correspond to the so-called M epitope of the Brucella O-antigen. This epitope is characterized by an a-(1^3)-linkage between D-perosamine units and is unique to Brucella. Here we report on an efficient approach to the synthesis of oligosaccharides that model the M epitope of the Brucella O-polysaccharide. The approach is based on the use of the a-(1^3)-linked disaccharide thioglycoside as the key donor block. Its application allowed the straightforward assembly of a set of four protected oligosaccharides, which includes a disaccharide, two trisaccharides, and a tetrasaccharide, in five glycosylation steps. The synthesized oligosaccharides are planned to be used in the development of diagnostic tools for identifying brucellosis in humans and domestic animals, as well as a potential vaccine against it. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22962646
Database :
Complementary Index
Journal :
Frontiers in Chemistry
Publication Type :
Academic Journal
Accession number :
178309155
Full Text :
https://doi.org/10.3389/fchem.2024.1424157