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Ferritin Nanoparticle Delivery of the E2 Protein of Classical Swine Fever Virus Completely Protects Pigs from Lethal Challenge.

Authors :
Zhong, Dailang
Lu, Zhanhao
Xia, Yu
Wu, Hongxia
Zhang, Xinyu
Li, Mingzhi
Song, Xin
Wang, Yanjin
Moon, Assad
Qiu, Hua-Ji
Li, Yongfeng
Sun, Yuan
Source :
Vaccines; Jun2024, Vol. 12 Issue 6, p629, 18p
Publication Year :
2024

Abstract

Classical swine fever (CSF), caused by the classical swine fever virus (CSFV), results in significant economic losses to the swine industry in many countries. Vaccination represents the primary strategy to control CSF and the CSFV E2 protein is known as the major protective antigen. However, the E2 protein expressed or presented by different systems elicits distinct immune responses. In this study, we established a stable CHO cell line to express the E2 protein and delivered it using self-assembled ferritin nanoparticles (NPs). Subsequently, we compared the adaptive immune responses induced by the E2-ferritin NPs and the monomeric E2 protein produced by the CHO cells or a baculovirus expression system. The results revealed that the NP-delivered E2 protein elicited higher titers of neutralizing antibodies than did the monomeric E2 protein in pigs. Importantly, only the NP-delivered E2 protein significantly induced CSFV-specific IFN-γ-secreting cells. Furthermore, all the pigs inoculated with the E2-ferritin NPs were completely protected from a lethal CSFV challenge infection. These findings demonstrate the ability of the E2-ferritin NPs to protect pigs against the lethal CSFV challenge by eliciting robust humoral and cellular immune responses. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2076393X
Volume :
12
Issue :
6
Database :
Complementary Index
Journal :
Vaccines
Publication Type :
Academic Journal
Accession number :
178194447
Full Text :
https://doi.org/10.3390/vaccines12060629