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Single-Cell Screening through Cell Encapsulation in Photopolymerized Gelatin Methacryloyl.
- Source :
- Micro (2673-8023); Jun2024, Vol. 4 Issue 2, p295-304, 10p
- Publication Year :
- 2024
-
Abstract
- This study evaluated the potential of gelatin methacryloyl (GelMA) for single-cell screening compared to polyethylene glycol diacrylate (PEGDA). GelMA photopolymerized at 1000–2000 mJ/cm<superscript>2</superscript> produced consistent patterns and supported HeLa cell viability. GelMA (5%w/v) facilitated better cell collection within 2 days due to its shape retention. GelMA demonstrated biocompatibility with HeLa cells exhibiting exponential proliferation and biodegradation over 5 days. The average cell displacement over 2 days was 16 µm. Two targeted cell recovery strategies using trypsin were developed: one for adherent cells encapsulated at 800 mJ/cm<superscript>2</superscript>, and another for floating cells encapsulated at 800 mJ/cm<superscript>2</superscript>, enabling the selective removal of unwanted cells. These findings suggest GelMA as a promising biomaterial for single-cell screening applications, offering advantages over PEGDA in cell encapsulation and targeted recovery. [ABSTRACT FROM AUTHOR]
- Subjects :
- PHOTOPOLYMERIZATION
GELATIN
POLYETHYLENE glycol
BIOCOMPATIBILITY
HELA cells
Subjects
Details
- Language :
- English
- ISSN :
- 26738023
- Volume :
- 4
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Micro (2673-8023)
- Publication Type :
- Academic Journal
- Accession number :
- 178183882
- Full Text :
- https://doi.org/10.3390/micro4020018