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Continuous chiral separation process for high‐speed countercurrent chromatography established and scaled up: A case of Voriconazole enantioseparation.
- Source :
- Journal of Separation Science; Jun2024, Vol. 47 Issue 12, p1-9, 9p
- Publication Year :
- 2024
-
Abstract
- An efficient method for the continuous separation of Voriconazole enantiomers was developed using sulfobutyl ether‐β‐cyclodextrin (SBE‐β‐CD) as a chiral selector in high‐speed countercurrent chromatography (HSCCC) with different types. The separation was performed using a two‐phase solvent system consisting of n‐hexane/ethyl acetate/100 mmol/L phosphate buffer solution (pH = 3.0, containing 50 mmol/L SBE‐β‐CD) (1.5:0.5:2, v/v/v). A fast and predictable scale‐up process was achieved using an analytical DE HSCCC instrument. The optimized parameters were subsequently applied to a preparative Tauto HSCCC instrument, resulting in consistent separation time and enantiomeric purity, with throughput boosted by a remarkable 11‐fold. Preparative HSCCC successfully separated 506 mg of the racemate, delivering enantiomers exceeding 99% purity as confirmed by high‐performance liquid chromatography analysis. This investigation presents an effective methodology for forecasting the HSCCC scale‐up process and attaining continuous separation of chiral drugs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16159306
- Volume :
- 47
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Journal of Separation Science
- Publication Type :
- Academic Journal
- Accession number :
- 178161300
- Full Text :
- https://doi.org/10.1002/jssc.202400190