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Plumieride as a novel anti-fungal and anti-inflammatory iridoid against superficial candidiasis in mice.
- Source :
- BMC Complementary Medicine & Therapies; 6/10/2024, Vol. 24 Issue 1, p1-12, 12p
- Publication Year :
- 2024
-
Abstract
- In the past few decades, there has been a notable rise in the occurrence of several types of candidiasis. Candida albicans is the most common cause of superficial fungal infections in humans. In this study, plumieride, one of the major iridoids from Plumeria obtusa L. leaves, was isolated and investigated for its potential against Candida albicans (CA)-induced dermatitis in mice. qRT-PCR was done to assess the impact of plumieride on the expression of the major virulence genes of CA. Five groups (n = 7) of adult male BALB/c mice were categorized into: group I: non-infected mice; group II: mice infected intradermally with 10<superscript>7</superscript>–10<superscript>8</superscript> CFU/mL of CA; group III: CA-infected mice treated with standard fluconazole (50 mg/kg bwt.); group IV and V: CA-infected mice treated with plumieride (25- and 50 mg/kg. bwt., respectively). All the treatments were subcutaneously injected once a day for 3 days. Skin samples were collected on the 4th day post-inoculation to perform pathological, microbial, and molecular studies. The results of the in vitro study proved that plumieride has better antifungal activity than fluconazole, manifested by a wider zone of inhibition and a lower MIC. Plumieride also downregulated the expression of CA virulence genes (ALS1, Plb1, and Hyr1). CA-infected mice showed extensive dermatitis, confirmed by strong iNOS, TNF-α, IL-1β, and NF-κB genes or immune expressions. Whereas the treatment of CA-infected mice with plumieride significantly reduced the microscopic skin lesions and modulated the expression of all measured proinflammatory cytokines and inflammatory markers in a dose-dependent manner. Plumieride interfered with the expression of C. albicans virulence factors and modulated the inflammatory response in the skin of mice infected with CA. [ABSTRACT FROM AUTHOR]
- Subjects :
- HETEROCYCLIC compounds
ANTIFUNGAL agents
ANTI-inflammatory agents
FLUCONAZOLE
IN vitro studies
NF-kappa B
HIGH performance liquid chromatography
SKIN inflammation
MICROBIAL virulence
DATA analysis
MICROBIAL sensitivity tests
POLYMERASE chain reaction
FUNGI
DESCRIPTIVE statistics
CELLULAR signal transduction
MANN Whitney U Test
PLANT extracts
CANDIDA albicans
MICE
GENES
GENE expression
DOSE-effect relationship in pharmacology
IMMUNOHISTOCHEMISTRY
MEDICINAL plants
ANIMAL experimentation
MOLECULAR structure
HISTOLOGICAL techniques
ANALYSIS of variance
STATISTICS
LEAVES
CANDIDIASIS
COMPARATIVE studies
NITRIC-oxide synthases
CYTOKINES
DATA analysis software
BIOMARKERS
SUBCUTANEOUS injections
TUMOR necrosis factors
INTERLEUKINS
MOLECULAR pathology
MICROBIAL genetics
NONPARAMETRIC statistics
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 178046312
- Full Text :
- https://doi.org/10.1186/s12906-024-04508-z