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In vitro and in vivo anti-parasitic activity of curcumin nanoemulsion on Leishmania major (MRHO/IR/75/ER).
- Source :
- BMC Complementary Medicine & Therapies; 6/18/2024, Vol. 24 Issue 1, p1-8, 8p
- Publication Year :
- 2024
-
Abstract
- The present study aimed to assess the anti-leishmanial effects of curcumin nanoemulsion (CUR-NE) against Leishmania major (MRHO/IR/75/ER) in both in vitro and in vivo experiments. CUR-NE was successfully prepared via the spontaneous emulsification method. The in vitro effect of various concentrations of CUR-NE against L. major promastigotes was assessed using the flow cytometry method. In vivo experiments were carried out in BALB/c mice inoculated subcutaneously with 2 × 10<superscript>6 </superscript>L. major promastigotes. Mice were treated with topical CUR-NE (2.5 mg/ml), intra-lesion injection of CUR-NE (2.5 mg/ml), topical CUR suspension (CUR-S, 2.5 mg/ml), topical NE without CUR (NE-no CUR), amphotericin B as the positive control group, and infected untreated mice as the negative control group. In vitro exposure of promastigotes to CUR-NE showed a dose-dependent anti-leishmanial effect, with a 67.52 ± 0.35% mortality rate at a concentration of 1250 µg/ml and an IC50 of 643.56 µg/ml. In vivo experiments showed that topical CUR-NE and CUR-S significantly decreased the mean lesion size in mice after four weeks from 4.73 ± 1.28 to 2.78 ± 1.28 mm and 4.45 ± 0.88 to 3.23 ± 0.59 mm, respectively (p = 0.001). Furthermore, CUR-NE significantly decreased the parasite load in treated mice compared with the negative control group (p = 0.001). Results from the current study demonstrated the promising activity of CUR-NE against L. major in both in vitro and in vivo experiments. Moreover, CUR-NE was more efficient than CUR-S in healing and reducing parasite burden in mouse models. Future studies should aim to identify molecular mechanisms as well as the pharmacologic and pharmacokinetic aspects of CUR-NE. [ABSTRACT FROM AUTHOR]
- Subjects :
- IN vitro studies
FLOW cytometry
EMULSIONS
MICROBIAL sensitivity tests
RESEARCH funding
PHARMACEUTICAL chemistry
KRUSKAL-Wallis Test
IN vivo studies
DESCRIPTIVE statistics
MANN Whitney U Test
LEISHMANIA
MICE
DOSE-effect relationship in pharmacology
CURCUMIN
DRUG efficacy
ANIMAL experimentation
DATA analysis software
LEISHMANIASIS
NANOPARTICLES
ANTIPARASITIC agents
REGRESSION analysis
PHARMACODYNAMICS
EVALUATION
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 177963472
- Full Text :
- https://doi.org/10.1186/s12906-024-04522-1