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Simplicispira sedimenti sp. nov., isolated from a sediment of drainage ditch in winery.
- Source :
- Antonie van Leeuwenhoek; 1/3/2024, Vol. 117 Issue 1, p1-10, 10p
- Publication Year :
- 2024
-
Abstract
- A Gram-stain-negative, motile (by single polar flagellum) and rod-shaped bacterium, designated W1-6<superscript>T</superscript>, was isolated from a sediment of drainage ditch in winery in Guiyang, south-western China. Strain W1-6<superscript>T</superscript> showed the highest 16S rRNA gene sequence similarities with the type strain of Acidovorax wautersii (98.1%) and Simplicispira lacusdiani (97.9%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W1-6<superscript>T</superscript> was placed adjacent to the members of the genus Simplicispira and formed a separat subclade. Cells showed oxidase and catalase negative reactions. The only respiratory quinone detected was ubiquinone-8 (Q-8). Summed feature 3 (C<subscript>16:1</subscript>ω7c and/or C<subscript>16:1</subscript>ω6c), C<subscript>16:0</subscript> and summed feature 8 (C<subscript>18:1</subscript>ω7c and/or C<subscript>18:1</subscript>ω6c) were predominant cellular fatty acids (> 10%) of strain W1-6<superscript>T</superscript>. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and five unidentified phospholipids were found in the polar lipid extraction. The genomic DNA G + C content was 65.6%. Strain W1-6<superscript>T</superscript> shared the highest digital DNA-DNA hybridization [dDDH, (27.6%)] and average nucleotide identity [ANI (84.3%)] values with the type strain of S. lacusdiani. The dDDH and ANI values were below the cutoff level (dDDH 70%; ANI 95–96%) for species delineation. The polyphasic characteristics indicated that the strain W1-6<superscript>T</superscript> represents a novel species of the genus Simplicispira, for which the name Simplicispira sedimenti sp. nov. is proposed. The type strain is W1-6<superscript>T</superscript> (= CGMCC 1.16274<superscript>T</superscript> = NBRC 115624<superscript>T</superscript>). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00036072
- Volume :
- 117
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 177878368
- Full Text :
- https://doi.org/10.1007/s10482-023-01899-w