Platelet rich fibrin incorporation into synthetic coral scaffold between drop and dip method.

In dentistry, oral and maxillofacial bone loss is often encountered due to diseases such as bone removal during tumor surgery and periapical bone damage due to periapical periodontitis. Regeneration is needed in tissue engineering with a good scaffold design according to tissue needs. The synthetic coral scaffold is a supplement with a base of gelatin and calcium carbonate (CaCO3). The scaffold is then incorporated with Platelet Rich Fibrin (PRF) which causes PRF to be activated to produce growth factors. There is a need for an effective method for incorporating PRF into the scaffolds. However, it is still unclear which method is more effective. This research aims to determine the ability of synthetic coral scaffold materials to be incorporated with PRF. The research design was an experimental laboratory study with a post-test design. The research subjects included 6 artificial coral scaffolds and 3 students of Universitas Muhammadiyah Yogyakarta to have their blood tested. The blood sample was then processed with PRF, and the number of platelets was counted using Giemsa staining. PRF would be dipped and dripped on synthetic coral scaffolds. The results of PRF incorporation on the synthetic coral scaffold were calculated utilizing the initial platelet count minus the number of platelets that had been dipped and dropped. Statistical tests using the Independent Sample t-Test obtained a value of p=0.00 (p<0.05), indicating a difference in PRF incorporation on the synthetic coral scaffolds between the dip and drop methods. As a result, there was a significant difference between the dip and drop methods in the PRF incorporation on synthetic coral scaffolds. [ABSTRACT FROM AUTHOR]