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Comparative transcriptomics revealed the mechanism of Stenotrophomonas rhizophila JC1 response and biosorption to Pb2+.
- Source :
- Environmental Geochemistry & Health; Jul2024, Vol. 46 Issue 7, p1-18, 18p
- Publication Year :
- 2024
-
Abstract
- Nowadays, there is limited research focusing on the biosorption of Pb<superscript>2+</superscript> through microbial process, particularly at the level of gene expression. To overcome this knowledge gap, we studied the adsorption capacity of Stenotrophomonas rhizophila JC1 to Pb<superscript>2+</superscript>, and investigated the physiological mechanism by means of SEM, EDS, FTIR, membrane permeability detection, and investigated the molecular mechanism through comparative transcriptomics. The results showed that after 16 h of cultivation, the biosorption capacity of JC1 for 100 mg/L of Pb<superscript>2+</superscript> reached at 79.8%. The main mechanism of JC1 adsorb Pb<superscript>2+</superscript> is via intracellular accumulation, accounting for more than 90% of the total adsorption. At the physiological level, Pb<superscript>2+</superscript> can precipitate with anion functional groups (e.g., –OH, –NH) on the bacterial cell wall or undergo replacement reaction with cell component elements (e.g., Si, Ca) to adsorb Pb<superscript>2+</superscript> outside of the cell wall, thus accomplishing extracellular adsorption of Pb<superscript>2+</superscript> by strains. Furthermore, the cell membrane acts as a “switch” that inhibits the entry of metal ions into the cell from the plasma membrane. At the molecular level, the gene pbt specificity is responsible for the adsorption of Pb<superscript>2+</superscript> by JC1. In addition, phosphate permease is a major member of the ABC transporter family involved in Pb<superscript>2+</superscript>, and czcA/cusA or Co<superscript>2+</superscript>/Mg<superscript>2+</superscript> efflux protein plays an important role in the efflux of Pb<superscript>2+</superscript> in JC1. Further, cellular macromolecule biosynthesis, inorganic cation transmembrane transport, citrate cycle (TCA) and carbon metabolism pathways all play crucial roles in the response of strain JC1 to Pb<superscript>2+</superscript> stress. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02694042
- Volume :
- 46
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Environmental Geochemistry & Health
- Publication Type :
- Academic Journal
- Accession number :
- 177764320
- Full Text :
- https://doi.org/10.1007/s10653-024-02019-x