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Magnetic Seeding of SPIO-BMSCs Into a Biphasic Scaffold Can Promote Tendon-Bone Healing After Rotator Cuff Repair.
- Source :
- American Journal of Sports Medicine; Jun2024, Vol. 52 Issue 7, p1707-1718, 12p
- Publication Year :
- 2024
-
Abstract
- Background: The tendon-bone interface (TBI) in the rotator cuff has a poor intrinsic capacity for healing, which increases the risk of retear after rotator cuff repair (RCR). However, facilitating regeneration of the TBI still remains a great clinical challenge. Herein, the authors established a novel strategy based on magnetic seeding to enhance the TBI regeneration. Hypothesis: Magnetic seeding bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide (SPIO-BMSCs) into a biphasic scaffold can promote tendon-bone healing after RCR. Study Design: Controlled laboratory study. Methods: BMSCs were labeled with SPIOs. Prussian blue staining, CCK-8 tests, Western blot, and quantitative reverse transcription polymerase chain reaction (PCR) were used to determine the optimal effect concentration of SPIOs on cell bioactivities and abilities. Then SPIO-BMSCs were magnetically seeded into a biphasic scaffold under a magnetic field. The seeding efficacy was assessed by a scanning electron microscope, and the potential mechanism in chondrogenic differentiation after seeding SPIO-BMSCs into the scaffold was evaluated by Western blot and PCR. Furthermore, the effect of SPIO-BMSC/biphasic scaffold on tendon-bone healing after RCR using a rat model was examined using histological analysis, enzyme-linked immunosorbent assay, and biomechanical evaluation. Results: BMSCs labeled with 100 μg/mL SPIO had no effect on cell bioactivities and the ability of chondrogenic differentiation. SPIO-BMSCs were magnetically seeded into a biphasic scaffold, which offered a high seeding efficacy to enhance chondrogenic differentiation of SPIO-BMSCs via the CDR1as/miR-7/FGF2 pathway for TBI formation in vitro. Furthermore, in vivo application of the biphasic scaffold with magnetically seeded SPIO-BMSCs showed their regenerative potential, indicating that they could significantly accelerate and promote TBI healing with superior biomechanical properties after RCR in a rat rotator cuff tear model. Conclusion: Magnetically seeding SPIO-BMSCs into a biphasic scaffold enhanced seeding efficacy to promote cell distribution and condensation. This construct enhanced the chondrogenesis process via the CDR1as/miR-7/FGF2 pathway and further promoted tendon-bone healing after RCR in a rat rotator cuff tear model. Clinical Relevance: This study provides an alternative strategy for improving TBI healing after RCR. [ABSTRACT FROM AUTHOR]
- Subjects :
- IRON oxide nanoparticles
IN vitro studies
BONE regeneration
ARTHROSCOPY
MESENCHYMAL stem cells
POLYMERASE chain reaction
ENZYME-linked immunosorbent assay
TREATMENT effectiveness
IN vivo studies
ROTATOR cuff
ORTHOPEDIC surgery
RATS
ROTATOR cuff injuries
TISSUE scaffolds
ANIMAL experimentation
WESTERN immunoblotting
SCANNING electron microscopy
HISTOLOGICAL techniques
CHONDROGENESIS
STAINS & staining (Microscopy)
Subjects
Details
- Language :
- English
- ISSN :
- 03635465
- Volume :
- 52
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- American Journal of Sports Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 177595331
- Full Text :
- https://doi.org/10.1177/03635465241247288