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Lysosomal-targeted fluorescent probe based pH regulating reactivity for tracking cysteine dynamics under oxidative stress.
- Source :
- Journal of Materials Chemistry B; 6/7/2024, Vol. 12 Issue 21, p5157-5161, 5p
- Publication Year :
- 2024
-
Abstract
- The ability to detect and visualize cellular events and associated biological analytes is essential for the understanding of their physiological and pathological functions. Cysteine (Cys) plays a crucial role in biological systems and lysosomal homeostasis. This puts forward higher requirements on the performance of the probe. Herein, we rationally designed a coumarin-based probe for the reversible, specific, sensitive, and rapid detection of Cys based on pH regulating reactivity. The obtained probe (ECMA) introduces a morpholine moiety to target lysosomes, and a,b-unsaturated-ketone with an electron-withdrawing CN group served as a reversible reaction site for Cys. Importantly, ECMA was successfully applied to the real-time monitoring of Cys dynamics in living cells. Furthermore, cell imaging clearly revealed that exogenous Cys could induce the up-regulation of lysosomal ROS, which provided a powerful tool for investigating the relationship between oxidative stress and lysosomal Cys. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2050750X
- Volume :
- 12
- Issue :
- 21
- Database :
- Complementary Index
- Journal :
- Journal of Materials Chemistry B
- Publication Type :
- Academic Journal
- Accession number :
- 177583854
- Full Text :
- https://doi.org/10.1039/d4tb00478g