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Enzymatic C1 reduction using hydrogen in cofactor regeneration.

Authors :
Sun, Ruishuang
Cao, Chenqi
Wang, Qingyun
Cao, Hui
Schwaneberg, Ulrich
Ji, Yu
Liu, Luo
Xu, Haijun
Source :
Frontiers of Chemical Science & Engineering; Jul2024, Vol. 18 Issue 7, p1-8, 8p
Publication Year :
2024

Abstract

Carbon dioxide fixation presents a potential solution for mitigating the greenhouse gas issue. During carbon dioxide fixation, C1 compound reduction requires a high energy supply. Thermodynamic calculations suggest that the energy source for cofactor regeneration plays a vital role in the effective enzymatic C1 reduction. Hydrogenase utilizes renewable hydrogen to achieve the regeneration and supply cofactor nicotinamide adenine dinucleotide (NADH), providing a driving force for the reduction reaction to reduce the thermodynamic barrier of the reaction cascade, and making the forward reduction pathway thermodynamically feasible. Based on the regeneration of cofactor NADH by hydrogenase, and coupled with formaldehyde dehydrogenase and formolase, a favorable thermodynamic mode of the C1 reduction pathway for reducing formate to dihydroxyacetone (DHA) was designed and constructed. This resulted in accumulation of 373.19 µmol·L<superscript>−1</superscript> DHA after 2 h, and conversion reaching 7.47%. These results indicate that enzymatic utilization of hydrogen as the electron donor to regenerate NADH is of great significance to the sustainable and green development of bio-manufacturing because of its high economic efficiency, no by-products, and environment-friendly operation. Moreover, formolase efficiently and selectively fixed the intermediate formaldehyde (FALD) to DHA, thermodynamically pulled formate to efficiently reduce to DHA, and finally stored the low-grade renewable energy into chemical energy with high energy density. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20950179
Volume :
18
Issue :
7
Database :
Complementary Index
Journal :
Frontiers of Chemical Science & Engineering
Publication Type :
Academic Journal
Accession number :
177525071
Full Text :
https://doi.org/10.1007/s11705-024-2431-3