Comparative Study on the Determination of Chlorophyll- a in Lake Phytoplankton by a YSI Multi-Parameter Water Quality Meter and Laboratory Spectrophotometric Method.

Algal blooms caused by eutrophication are a major global problem, and the monitoring and prediction of algal densities in lakes are important indicators of eutrophication management. However, the reliability of the commonly used chlorophyll-a (Chl-a) to characterize phytoplankton density in lake environments needs to be further investigated. In this paper, we sampled and analyzed 365 samples from nine plateau lakes in Yunnan Province during the dry and rainy seasons. The Chl-a data measured by the laboratory spectrophotometric method and the portable YSI multi-parameter water quality meter (YSI) directly used in the field were compared, and regression analysis and correlation analysis with phytoplankton density were performed. Most of the Chl-a values measured by the laboratory instrument were greater than those measured by the YSI, and the correlation between the two methods was weak (0.492, p < 0.001). The correlation between Chl-a and phytoplankton density measured by the YSI reached 0.67 (p < 0.001) in the dry season, while the laboratory methods used to measure Chl-a to characterize phytoplankton density may have led to an overestimation of phytoplankton density due to nonspecific sources of Chl-a. However, both methods are relatively inaccurate for characterizing phytoplankton density. For different trophic states of lakes, nutrient concentration changes affect the Chl-a concentration of phytoplankton. During different seasons, changes in the fluorescence intensity of phytoplankton in response to environmental conditions prevent the YSI results from reflecting the authentic phytoplankton density. Furthermore, high species diversity can lead to inconsistent changes in Chl-a and phytoplankton because the content of Chl-a in individual cells of different phytoplankton is different. The relationship between Chl-a and phytoplankton density was species specific. Therefore, when applying Chl-a to characterize phytoplankton density in lakes, it is necessary to consider environmental conditions, phytoplankton community structure and other practical conditions. In addition, laboratory analytical methods and instrumental techniques and instruments need to be improved. [ABSTRACT FROM AUTHOR]