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Morphological, Molecular Structural and Physicochemical Characterization of Starch Granules Formed in Endosperm of Rice with Ectopic Overexpression of α-Amylase.
- Source :
- Journal of Applied Glycoscience; 2024, Vol. 71 Issue 1, p23-32, 10p
- Publication Year :
- 2024
-
Abstract
- The objective of this study was to characterize the endosperm starch in rice that ectopically overexpressed the α-amylase. Transgenic rice plants, transformed with cauliflower mosaic virus 35S promoter driven AmyI-1 (35S::AmyI-1) and AmyII-4 (35S::AmyII-4), and 10 kDa prolamin promoter driven AmyI-1 (P10::AmyI-1), were cultivated under standard conditions (23 °C, 12 h in the dark/ 26 °C, 12 h in the light), and brown grains were subsequently harvested. Each grain displayed characteristic chalkiness, while electron microanalyzer (EPMA)-SEM images disclosed numerous small pits on the surface of the starch granules, attributable to α-amylase activity. Fluorescence labeling and capillary electrophoresis analysis of starch chain length distribution revealed no significant alterations in the starches of 35S::AmyI-1 and 35S::AmyII-4 transgenic rice compared to the wild-type. Conversely, the extremely short α-glucan chains (DP 2-8) exhibited a dramatic increase in the P10::AmyI-1 starch. Rapid visco-analyzer analysis also identified variations in the chain length distribution of P10::AmyI-1 starch, manifesting as changes in viscosity. Moreover, ¹H-NMR analysis uncovered dynamic modifications in the molecular structure of starch in rice grain transformed with P10::AmyI-1, which was found to possess unprecedented structural characteristics. [ABSTRACT FROM AUTHOR]
- Subjects :
- AMYLOPLASTS
ENDOSPERM
RICE
AMYLASES
CHAIN length (Chemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 13447882
- Volume :
- 71
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Applied Glycoscience
- Publication Type :
- Academic Journal
- Accession number :
- 177443144
- Full Text :
- https://doi.org/10.5458/jag.jag.JAG-2023_0016