Back to Search
Start Over
Enhanced immunomodulation and periodontal regeneration efficacy of subgingivally delivered progranulin‐loaded hydrogel as an adjunct to non‐surgical treatment for Class II furcation involvement in dogs.
- Source :
- Journal of Clinical Periodontology; Jun2024, Vol. 51 Issue 6, p774-786, 13p
- Publication Year :
- 2024
-
Abstract
- Aim: To evaluate the effect of subgingival delivery of progranulin (PGRN)/gelatin methacryloyl (GelMA) complex as an adjunct to scaling and root planing (SRP) on an experimental periodontitis dog model with Class II furcation involvement (FI). Materials and Methods: A Class II FI model was established, and the defects were divided into four treatment groups: (a) no treatment (control); (b) SRP; (c) SRP + GelMA; (d) SRP + PGRN/GelMA. Eight weeks after treatment, periodontal parameters were recorded, gingival crevicular fluid and gingival tissue were collected for ELISA and RT‐qPCR, respectively, and mandibular tissue blocks were collected for micro computed tomography (micro‐CT) scanning and hematoxylin and eosin (H&E) staining. Results: The SRP + PGRN/GelMA group showed significant improvement in all periodontal parameters compared with those in the other groups. The expression of markers related to M1 macrophage and Th17 cell significantly decreased, and the expression of markers related to M2 macrophage and Treg cell significantly increased in the SRP + PGRN/GelMA group compared with those in the other groups. The volume, quality and area of new bone and the length of new cementum in the root furcation defects of the PGRN/GelMA group were significantly increased compared to those in the other groups. Conclusions: Subgingival delivery of the PGRN/GelMA complex could be a promising non‐surgical adjunctive therapy for anti‐inflammation, immunomodulation and periodontal regeneration. [ABSTRACT FROM AUTHOR]
- Subjects :
- PERIODONTITIS treatment
PERIODONTAL disease treatment
BIOLOGICAL models
DENTAL scaling
ANTI-inflammatory agents
RESEARCH funding
MACROPHAGES
GINGIVA
ENZYME-linked immunosorbent assay
GUIDED tissue regeneration
DRUG delivery systems
PHARMACEUTICAL gels
TREATMENT effectiveness
IMMUNE system
DOGS
DESCRIPTIVE statistics
REVERSE transcriptase polymerase chain reaction
EXPERIMENTAL design
GENE expression
GROWTH factors
ANIMAL experimentation
TOOTH root planing
COLLECTION & preservation of biological specimens
STAINS & staining (Microscopy)
MANDIBLE
PERIODONTITIS
Subjects
Details
- Language :
- English
- ISSN :
- 03036979
- Volume :
- 51
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Journal of Clinical Periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 177219280
- Full Text :
- https://doi.org/10.1111/jcpe.13955