Technical challenges of studying the impact of plasma components on the efficacy of lipid nanoparticles for vaccine and therapeutic applications.

The article discusses the technical challenges of studying the impact of plasma components on the efficacy of lipid nanoparticles (LNPs) for vaccine and therapeutic applications. The authors question a method proposed by Liu et al. to isolate LNPs from plasma, expressing concerns about the potential co-isolation of lipoproteins and extracellular vesicles (EVs). They highlight the importance of understanding how LNPs interact with the biochemical environment they are intended to function in and suggest control experiments to verify the isolation method. The article emphasizes the need for further research to ensure accurate characterization of LNPs and their interactions with plasma constituents. [Extracted from the article]