Spatial Evaluation of the Heavy Metal Iron in Soil, Pond Water and its Mobility into the Muscles of Zebrafish using ICP-OES.

Background: The study has a bifold objectives, firstly to determine the concentration of iron (Fe) in the soil and pond water within the vicinity of a historical gold mine region located in the Kolar Gold fields (KGF), Karnataka, India. Secondly, the study seeks to investigate the process of bioaccumulation of the heavy metal, iron in the muscles of Zebrafish inhabiting the same area. Methods: To achieve the objectives, the study employed Thermo Scientific's Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and ICP-AES using Iteva software to evaluate and statistically analyze the distribution of iron in the soil, pond water and muscles samples. Four soil sample sites (A, B, C and D) were selected based on their proximity to residential area. Soil samples were examined from January to December, 2022, while pond water samples were analyzed between April and October, 2022 following a southwest monsoon rain. Result: During the study, the analysis of soil samples taken from the four mine landfills revealed elevated levels of heavy metals, with the highest mean concentration of 41915.17 mg/kg found in Site A. In the pond water, sample site B exhibited an exceedingly high Fe concentration during October with 5.56 mg/L followed by sample site D, A and C with 4.89, 3.89 and 3.54 mg/L, respectively against 0.98 mg/L in the control site. The concentration of Fe in the muscles was exceedingly high in the sample site B with 10457 mg/Kg on day 30 of October to that of 8219 mg/Kg on day 30 of April month, which is far above the threshold value of 100 mg/kg as per the regulatory bodies (WHO 1993). The outcome of this experiment will facilitate the assessment of surface soil contamination levels and the likelihood of contaminants entering the aquatic environment, resulting in the bioaccumulation of heavy metals in the aquatic organisms. [ABSTRACT FROM AUTHOR]