Study of Human Osteosarcoma Cell Line Growth, Hemocompatibility, In-vitro Analysis and Physical Properties of V2O5 Substituted Borosilicate Glass.

Bioactive borosilicate glasses having weight composition (74-X) SiO₂, 16 Na₂O, 10 B₂O₃ and (X) V₂O₅ (wt%), where (X = 0, 0.1, 0.3, 0.5, 1, 2.5, 4 wt %) were synthesized using the conventional melting procedure. Bioactive glass samples were melted in a platinum crucible of 50 ml capacity at 1400 °C in the globular furnace with air as the furnace atmosphere and kept hold for 2 h. These glasses were characterised and tested by using several experiment techniques like XRD, FTIR, SEM and EDX. The bioactivity of the borosilicate samples was determined by submerging them in simulated body fluid (SBF) for different time intervals. The developed hydroxy apatite (HA) was identified by FTIR, XRD, SEM and EDX technique. The obtained results demonstrate that the in-vitro bioactivity of the samples increases with the increase in V₂O₅ doping in samples. Various properties like density, compressive strength and electrical properties are determined. Physical and mechanical properties are improved after V₂O₅ doping. Electrical properties are improved after SBF doping, and the result shows the ion movement due to increased bioactivity. The in-vitro hemocompatibility and cellular compatibility on MG-63 cell lines were carried out. It was found that samples were compatible with human blood. Cellular proliferation is increased with increasing concentration of V₂O₅. However beyond 0.5% V₂O₅ doping the value of bioactivity decreases at higher concentrations of samples in culture media. [ABSTRACT FROM AUTHOR]