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Comparison of Copper-Tolerance Genes between Different Groups of Acidovorax citrulli.

Authors :
Zhang, Min
Zhao, Mei
Qiao, Pei
Liu, Dehua
Bai, Qingrong
Guan, Wei
Yang, Yuwen
Zhao, Tingchang
Source :
Microorganisms; Apr2024, Vol. 12 Issue 4, p682, 13p
Publication Year :
2024

Abstract

Acidovorax citrulli populations exhibit genetic and phenotypic variations, particularly in terms of copper tolerance. Group I strains of A. citrulli generally exhibit higher copper tolerance compared to group II strains. This study aims to identify genes involved in copper tolerance to better understand the differences in copper tolerance between group I and group II strains. Representative strains pslb65 (group I) and pslbtw14 (group II) were selected for comparison. Deletion mutants of putative copper-tolerance genes and their corresponding complementary strains were constructed. The copper tolerance of each strain was evaluated using the minimum inhibitory concentration method. The results showed that the copA, copZ, cueR, and cueO genes played major roles in copper tolerance in A. citrulli, while cusC-like, cusA-like, and cusB-like genes had minor effects. The different expression levels of copper-tolerance-related genes in pslb65 and pslbtw14 under copper stress indicated that they had different mechanisms for coping with copper stress. Overall, this study provides insights into the mechanisms of copper tolerance in A. citrulli and highlights the importance of specific genes in copper tolerance. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20762607
Volume :
12
Issue :
4
Database :
Complementary Index
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
176907712
Full Text :
https://doi.org/10.3390/microorganisms12040682