CYCLIN D1 (G870A) POLYMORPHISM AND BREAST CANCER RISK IN GUILAN PROVINCE POPULATION OF IRAN.

Cyclins are the key regulator of the cell cycle, and their over-expression has been seen in many cancers, including breast cancer. Cyclin D1 is an oncoprotein encoded by the CCND1 gene located on chromosome 11 (11q) that regulates the cell cycle in shifting from the G1 to the S phase. It's the main target for steroids and mitogenic growth hormones in breast epithelial cells. This study aimed to evaluate the relationship between Cyclin D1 G870A polymorphism and breast cancer risk in a population of Guilan province in the north of Iran. Whole blood samples (CBC Tube) were collected from 82 patients with breast cancer and 66 healthy women. DNA extraction and genotyping were performed by Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) technique. Genotypic prevalence of AA, AG, and GG genotypes among patients were 40.2%, 35.3%, and 24.4%, and in controls were 30%, 47%, and 23%, respectively. There was no significant difference in CCND1 G870A genotype polymorphism between patients and the control group (p=0.32). Also, the allelic prevalence of A and G alleles in breast cancer patients was 58% and 42%, in controls were 54% and 46%, respectively. The present study showed that there is no significant association between CCND1 G870A polymorphism and the risk of breast cancer. The results of this study revealed that there is no significant association between CCND1 G870A genetic polymorphism and the risk of breast cancer in the population of Guilan province of Iran. More studies with larger samples of cases and controls would be beneficial. [ABSTRACT FROM AUTHOR]