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Study on the interaction between erythrosine B and the cardiac drug amiodarone using fluorescence, scattering, and absorbance spectra and their analytical application.

Authors :
AlSalem, Huda Salem
Alharbi, Sara Naif
Binkadem, Mona Saad
Mahmoud, Shimaa A.
Abdel‐Lateef, Mohamed A.
Source :
Luminescence: Journal of Biological & Chemical Luminescence; Apr2024, Vol. 39 Issue 4, p1-10, 10p
Publication Year :
2024

Abstract

In an acidic buffered solution, erythrosine B can react with amiodarone to form an association complex, which not only generates great enhancement in resonance Rayleigh scattering (RRS) spectrum of erythrosine B at 346.5 nm but also results in quenching of fluorescence spectra of erythrosine B at λemission = 550.4 nm/λexcitation = 528.5 nm. In addition, the formed erythrosine B–amiodarone complex produces a new absorbance peak at 555 nm. The spectral characteristics of the RRS, absorbance, and fluorescence spectra, as well as the optimum analytical conditions, were studied and investigated. As a result, new spectroscopic methods were developed to determine amiodarone by utilizing erythrosine B as a probe. Moreover, the ICH guidelines were used to validate the developed RRS, photometric, and fluorimetric methods. The enhancements in the absorbance and the RRS intensity and the decrease in the fluorescence intensity of the used probe were proportional to the concentration of amiodarone in ranges of 2.5–20.0, 0.2–2.5, and 0.25–1.75 μg/mL, respectively. Furthermore, limit of detection values were 0.52 ng/mL for the spectrophotometric method, 0.051 μg/mL for the RRS method, and 0.075 μg/mL for the fluorimetric method. Moreover, with good recoveries, the developed spectroscopic procedures were applied to analyze amiodarone in its commercial tablets. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15227235
Volume :
39
Issue :
4
Database :
Complementary Index
Journal :
Luminescence: Journal of Biological & Chemical Luminescence
Publication Type :
Academic Journal
Accession number :
176846261
Full Text :
https://doi.org/10.1002/bio.4748