The impact of mutual interactions between ZIFs-MWCNTs and hemoglobin molecule on electrocatalysis on hydrogen peroxide reduction of integrated heme protein.

This study proposes a composite consisting of zinc-heteroaromatic chemical framework material and hollow carbon-based material, designed to host hemoglobin. A heme protein-based electrode is developed using the complex with protein entrapment. The competitive coordination of heme protein with carbon-based carrier results in a novel intermediate with distinctive spectrometric and electrochemical features. The interaction between redox protein supporter and heme protein would give birth to the predominant influences the configuration of the cofactor within the protein. The article elucidates the role of the intermediate species in heme protein-induced electrocatalysis. Two simultaneous redox processes occur on the heme protein-based electrode. The intermediate species exhibits the favorable heterogeneous electron transfer dynamics (the normalized rate constant: 4.2 s⁻¹). The analysis in the enzyme-involved electro-catalysis suggests that the substrate diffusion dominates the sensing efficiency of the electrochemical sensor. [ABSTRACT FROM AUTHOR]