Effects of Chlortetracycline and Oxytetracycline on Microorganisms in Biological Phosphorus Removal.

To reveal the effect of chlortetracycline and oxytetracycline on microorganisms in biological phosphorus removal system, three mixtures of chlortetracycline and oxytetracycline with different ratios (R1, R2 and R3) were designed by direct equipartition radiography. The effects of three mixtures on microorganisms were analyzed by high-throughput sequencing technology, the correlation between environmental factors and microorganisms was analyzed by RStudio, and the interrelationship between microorganisms was visualized with R-4.1.0 software and Gephi. The results show that when the ratio is R1, microorganisms are more sensitive and significant in response to environmental factors. When the ratio is R2, the correlation between microbial structure is more stable, and the correlation between microorganisms is closer. The abundance of biological phosphorus removal bacteria decreased under the three ratios. Among them, the abundance of Acinetobacter decreased most significantly at R3, with a decrease of 36.11%. Research showed that there were significant differences in the effects of different ratios of chlortetracycline and oxytetracycline on microorganisms during biological phosphorus removal systems. [ABSTRACT FROM AUTHOR]